Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure
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Pyruvate carboxylase (PC) fromBacillus thermodenitrificanswas engineered in such away that the polypeptide chainwasdivided into two, between the biotin carboxylase (BC) andcarboxyl transferase (CT) domains. The two proteins thusformed, PC-(BC) and PC-(CT+BCCP), retained theircatalytic activity as assayed by biotin-dependent ATPaseand oxamate-dependent oxalacetate decarboxylation, forthe former and the latter, respectively. Neither activity wasdependent on acetyl-CoA, in sharp contrast to the completereaction of intact PC. ...
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Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure
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Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure
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