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Two isoforms of a heme oxygenase gene,ho1andho2, with 51% identityin amino acid sequence have been identified in the cyanobacteriumSynechocystissp. PCC 6803. Isoform-1, Syn HO-1, has been characterized,while isoform-2, Syn HO-2, has not. In this study, a full-lengthho2genewas cloned using synthetic DNA and Syn HO-2 was demonstrated to behighly expressed inEscherichia colias a soluble, catalytically active protein.Like Syn HO-1, the purified Syn HO-2 bound hemin stoichiometrically toform a heme–enzyme complex and degraded heme to biliverdin IXa,COand iron in the presence of reducing systems such as NADPH⁄ferredoxinreductase⁄ferredoxin and sodium ascorbate. ...
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Protein expressed by the ho2 gene of the cyanobacterium Synechocystis sp. PCC 6803 is a true heme oxygenase
