Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai
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A cellulase [endo-b-1,4-D-glucanase (EC 3.2.1.4)] was iso-lated from the hepatopancreas of abalone Haliotis discushannaiby successive chromatographies on TOYOPEARLCM-650M, hydroxyapatite and Sephacryl S-200 HR. Themolecular mass of the cellulase was estimated to be66 000 Da by SDS/PAGE, thus the enzyme was namedHdEG66. The hydrolytic activity of HdEG66 toward carb-oxymethylcellulose showed optimal temperature and pH at38Cand 6.3, respectively.
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Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai
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Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai
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