Báo cáo khoa học: Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana
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The present study reports the first purification and kineticcharacterization of two plant arogenate dehydrogenases(EC 1.3.1.43), an enzyme that catalyses the oxidativedecarboxylation of arogenate into tyrosine in presence ofNADP. The twoArabidopsis thalianaarogenate dehy-drogenases TyrAAT1 and TyrAAT2 were overproducedin Escherichia coliand purified to homogeneity. Bio-chemical comparison of the two forms revealed that atlow substrate concentration TyrAAT1 is four times moreefficient in catalyzing the arogenate dehydrogenase reac-tion than TyrAAT2. ...
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Báo cáo khoa học: Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Purification and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana
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