Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris
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A cellulase (endo-b-1,4-glucanase, EC 3.2.1.4) was purifiedfrom the gut of larvae of the yellow-spotted longicorn beetlePsacothea hilarisby acetone precipitation and elution fromgels after native PAGE and SDS/PAGE with activitystaining. The purified protein formed a single band, and themolecular mass was estimated to be 47 kDa. The purifiedcellulasedegradedcarboxymethylcellulose (CMC), insolublecello-oligosaccharide (average degree of polymerization 34)and soluble cello-oligosaccharides longer than cellotriose,but not crystalline cellulose or cellobiose. ...
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris
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