báo cáo khoa học: Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library

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báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library"Journal of Experimental &Clinical Cancer Research This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide LibraryJournal of Experimental & Clinical Cancer Research 2011, 30:105 doi:10.1186/1756-9966-30-105 Xiangan Tu (txabs9988@163.com) Jintao Zhuang (brianzg86@163.com) Wenwei Wang (Wangww2000@yahoo.com) Liang Zhao (Liang2046@163.com) Liangyun Zhao (bytx1974@163.com) Jiquan Zhao (jiquanzhao2010@163.com) Chunhua Deng (dch0310@163.com) Shaopeng Qiu (Urology@vip.163.com) Yuanyuan Zhang (yzhang@wfubmc.edu) ISSN 1756-9966 Article type Research Submission date 30 May 2011 Acceptance date 10 November 2011 Publication date 10 November 2011 Article URL http://www.jeccr.com/content/30/1/105 This peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below).Articles in Journal of Experimental & Clinical Cancer Research are listed in PubMed and archived at PubMed Central. For information about publishing your research in Journal of Experimental & Clinical Cancer Research or any BioMed Central journal, go to http://www.jeccr.com/authors/instructions/ For information about other BioMed Central publications go to © 2011 Tu et al. ; licensee BioMed Central Ltd.This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Journal of Experimental &Clinical Cancer Research http://www.biomedcentral.com/ © 2011 Tu et al. ; licensee BioMed Central Ltd.This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Screening and Identification of a Renal Carcinoma Specific Peptide from aPhage Display Peptide LibraryXiangan Tu1*§, Jintao Zhuang1*, Wenwei Wang1, Liang Zhao1, Liangyun Zhao1,Jiquan Zhao1, Chunhua Deng1, Shaopeng Qiu1, Yuanyuan Zhang2§1Department of Urology, The First Affiliated Hospital, Sun Yat-sen University,Guangzhou 510700, Guangdong, PR China.2Wake Forest Institute for Regenerative Medicine, Wake Forest University HealthSciences, Winston-Salem, NC, 27157, USA.*These authors contributed equally to this work§Corresponding authorTXA: txabs9988@163.comZJT: brianzg86@163.comWWW:Wangww2000@yahoo.comZL: Liang2046@163.comZLY: bytx1974@163.comZJQ: jiquanzhao2010@163.comDCH:dch0310@163.comQSP: Urology@vip.163.comZYY: yzhang@wfubmc.edu 1AbstractBackground Specific peptide ligands to cell surface receptors have been extensivelyused in tumor research and clinical applications. Phage display technology is apowerful tool for the isolation of cell-specific peptide ligands. To screen and identifynovel markers for renal cell carcinoma, we evaluated a peptide that had beenidentified by phage display technology.Methods A renal carcinoma cell line A498 and a normal renal cell line HK-2 wereused to carry out subtractive screening in vitro with a phage display peptide library.After three rounds of panning, there was an obvious enrichment for the phagesspecifically binding to the A498 cells, and the output/input ratio of phages increasedabout 100 fold. A group of peptides capabl ...