Báo cáo khoa học: Searching for folded proteins in vitro and in silico
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Understanding the sequence determinants of protein struc-ture, stabilityand folding is critical for understanding hownatural proteins have evolved and how proteins can beengineeredtoperformnovel functions.Thecomplexityof theprotein folding problem requires the abilityto search largevolumes of sequence space for proteins with specific struc-tural or functional characteristics. Here we describe ourefforts to identifynovel proteins using a phage-displayselection strategyfrom amini-exon shuffling librarygener-ated from the yeast genome and from completely randomsequence libraries, and compare the results to recent succes-ses in generating novel proteins usingin silicoprotein design....
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Báo cáo khoa học: Searching for folded proteins in vitro and in silico
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Báo cáo khoa học: Searching for folded proteins in vitro and in silico
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