Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha
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The soluble, cytoplasmic NAD+-reducing [NiFe]-hydro-genase fromRalstonia eutrophais aheterotetrameric enzyme(HoxFUYH) and contains two FMN groups. The purifiedoxidized enzyme is inactive in the H2-NAD+reaction, butcan be activated by catalytic amounts of NADH. It wasdiscovered that one of the FMN groups (FMN-a) is selec-tively released upon prolonged reduction of the enzymewith NADH. During this process, the enzyme maintainedits tetrameric form, with one FMN group (FMN-b) firmlybound, but it lost its physiological activity – the reduction ofNAD+by H2. ...
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Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha
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Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha
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