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Báo cáo khoa học: Selectivity of pyruvate kinase for Na+ and K+ in water/dimethylsulfoxide mixtures

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In aqueous media, muscle pyruvate kinase is highlyselective for K+over Na+. We now studied the selec-tivity of pyruvate kinase in water/dimethylsulfoxide mix-tures by measuring the activation and inhibition constantsof K+and Na+, i.e. their binding to the monovalent anddivalent cation binding sites of pyruvate kinase, respect-ively [Melchoir J.B. (1965) Biochemistry4, 1518–1525]. In40% dimethylsulfoxide theK0.5 appfor K+and Na+were190 and 64-fold lower than in water.Kiappfor K+andNa+decreased 116 and 135-fold between 20 and 40%dimethylsulfoxide. The ratios of Kiapp/K0.5 app for K+and Na+were 34–3.5 and 3.3–0.2, respectively....
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Báo cáo khoa học: Selectivity of pyruvate kinase for Na+ and K+ in water/dimethylsulfoxide mixtures

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