Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution
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The tyrosine phenol-lyase fromSymbiobacterium toebiiwas engineered toimprove both its stability and catalytic activity by the application of ran-dom mutagenesis and subsequent reassembly of the acquired mutations.Activity screening of the random library produced four mutants with atwo-fold improved activity, whereas parallel screening after heat treatmentat 65C identified three mutants with half-inactivation temperaturesimproved by up to 5.6 C.
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution
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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution
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