Danh mục

Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA

Số trang: 13      Loại file: pdf      Dung lượng: 480.77 KB      Lượt xem: 5      Lượt tải: 0    
tailieu_vip

Xem trước 2 trang đầu tiên của tài liệu này:

Thông tin tài liệu:

The interaction of the homeodomain of the sunflower KNOX proteinHAKN1 with DNA was studied by site-directed mutagenesis, hydroxylradical footprinting and missing nucleoside experiments. Binding ofHAKN1 to different oligonucleotides indicated that HAKN1 prefers thesequence TGACA (TGTCA), with changes within the GAC core more pro-foundly affecting the interaction. Footprinting and missing nucleosideexperiments using hydroxyl radical cleavage of DNA showed that HAKN1interacts with a 6-bp region of the strand carrying the GAC core, coveringthe core and nucleotides towards the 3¢ end....
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA

Tài liệu được xem nhiều: