Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA
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The interaction of the homeodomain of the sunflower KNOX proteinHAKN1 with DNA was studied by site-directed mutagenesis, hydroxylradical footprinting and missing nucleoside experiments. Binding ofHAKN1 to different oligonucleotides indicated that HAKN1 prefers thesequence TGACA (TGTCA), with changes within the GAC core more pro-foundly affecting the interaction. Footprinting and missing nucleosideexperiments using hydroxyl radical cleavage of DNA showed that HAKN1interacts with a 6-bp region of the strand carrying the GAC core, coveringthe core and nucleotides towards the 3¢ end....
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Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA
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