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Expression of DNase II in macrophages is potentially cru-cially important in the removal of unwantedDNA.We havepreviously shown that DNase II expression is up-regulatedat the transcriptional level during the phorbol 12-myristate-13-acetate (PMA)-induced differentiation of HL-60 andTHP-1 cells. In this study, we investigated thecis-regulatoryelements and transcription factors involved in this process inHL-60 cells.cis-Regulatory elements in the DNase II pro-moter were located by 5¢ deletion and site-directed muta-genesis of promoter-luciferase constructs and transienttransfection of HL-60 cells....
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Sp1 and Sp3 are involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells
