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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides

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Trypanosoma cruzi, the parasitic protozoan that causes Chagas disease,contains a major cysteine proteinase, cruzipain. This lysosomal enzymebears an unusual C-terminal extension that contains a number of post-translational modifications, and most antibodies in natural and experimen-tal infections are directed against it. In this report we took advantage ofUV-MALDI-TOF mass spectrometry in conjunction with peptide N-gly-cosidase F deglycosylation and high performance anion exchange chroma-tography analysis to address the structure of the N-linked oligosaccharidespresent in this domain. ...
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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides

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