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High hydrostatic pressure induced cold inactivation ofcarboxypeptidase Y. Carboxypeptidase Y was fully activewhen exposed to subzero temperature at 0.1 MPa; however,the enzyme became inactive when high hydrostatic pressureand subzero temperature were both applied. When theenzyme was treated at pressures higher than 300 MPa andtemperatures lower than)5C, it underwent an irreversibleinactivation in which nearly 50% of the a-helical structurewas lost as judged by circular dichroism spectral analysis....
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Báo cáo khoa học: Sub-zero temperature inactivation of carboxypeptidase Y under high hydrostatic pressure
