Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase
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Proteolysis of single polypeptide mammalian purple acid phosphatases(PAPs) results in the loss of an interaction between the loop residueAsp146 and the active site residues Asn91 and⁄or His92. While Asn91 is aligand to the divalent metal of the mixed-valent di-iron center, the role ofHis92 in the catalytic mechanism is unknown. Site-directed mutagenesis ofHis92 was performed to examine the role of this residue in single polypep-tide PAP.
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Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase
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Báo cáo khoa học: Substrate positioning by His92 is important in catalysis by purple acid phosphatase
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Report medicine traditional medicine scientific reports medicine cell proliferation oxidationTài liệu liên quan:
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