Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides
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Important hydrogenbonding interactions between substrateOH-groups inyeasta-glucosidases andoligo-1,6-glucosidasefrom glycoside hydrolase family 13 have been identi®ed bymeasuring the rates of hydrolysis of methyla-isomaltosideand its seven monodeoxygenated analogs. The transition-state stabilization energy, DDGà, contributed by the indi-vidual OH-groups was calculated from the activities forthe parent and the deoxy analogs, respectively, accordingtoDDGà ±RTln[(Vmax/Km)analog/(Vmax/Km)parent]....
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Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides
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Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides
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