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p-Hydroxyphenylacetate decarboxylase from Clostridiumdifficilecatalyses the decarboxylation ofp-hydroxyphenyl-acetate to yield the cytotoxic compoundp-cresol. The threegenes encoding two subunits of the glycyl-radical enzymeand the activating enzyme have been cloned and expressedin Escherichia coli. The recombinant enzymes were usedto reconstitute a catalytically functional system in vitro.Incontrast with the decarboxylase purified fromC. difficile,whichwas an almost inactive homo-dimeric protein (b2), therecombinant enzyme was a hetero-octameric (b4c4), cata-lytically competent complex, ...
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Báo cáo khoa học: Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase
