Báo cáo khoa học: Tamavidins – novel avidin-like biotin-binding proteins from the Tamogitake mushroom
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Mycobacterium tuberculosis glutamyl–tRNA synthetase (Mt-GluRS),encoded by Rv2992c, was overproduced inEscherichia colicells, and puri-fied to homogeneity. It was found to be similar to the other well-character-ized GluRS, especially the E. colienzyme, with respect to the requirementfor bound tRNAGluto produce the glutamyl-AMP intermediate, and thesteady-state kinetic parameterskcat (130 min)1) and KMfor tRNA (0.7 lm)and ATP (78lm), but to differ by a one order of magnitude higher KMvalue forl-Glu (2.7 mm). ...
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Báo cáo khoa học: Tamavidins – novel avidin-like biotin-binding proteins from the Tamogitake mushroom
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Báo cáo khoa học: Tamavidins – novel avidin-like biotin-binding proteins from the Tamogitake mushroom
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