Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase
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We report the recombinant neurolysin and thimet oligo-peptidase (TOP) hydrolytic activities towards internallyquenched fluorescent peptides derived from the peptideAbz-GGFLRRXQ-EDDnp (Abz,ortho-aminobenzoicacid;EDDnp,N-(2,4-dinitrophenyl) ethylenediamine), in whichX was substituted by 11 different natural amino acids.Neurolysin hydrolyzed these peptides at R–R or at R–Xbonds, and TOP hydrolyzed at R–R or L–R bonds,showing a preference to cleave at three or four aminoacids from the C-terminal end. ...
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Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase
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Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase
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