Báo cáo khoa học: The calcium-induced switch in the troponin complex probed by fluorescent mutants of troponin I
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The Ca2+-induced transition in the troponin complex (Tn)regulates vertebrate striated muscle contraction. Tn wasreconstituted with recombinant forms of troponin I (TnI)containing a single intrinsic 5-hydroxytryptophan (5HW).Fluorescence analysis of these mutants of TnI demonstratethat the regions in TnI that respond to Ca2+binding tothe regulatoryN-domain of TnC are the inhibitoryregion(residues 96–116) and a neighboring region that includesposition 121. Our data confirms the role of TnI as amodulator of the Ca2+affinityof TnC; we show that pointmutations and incorporation of 5HWinTnI can affect boththe affinityand the cooperativityof Ca2+binding to TnC....
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Báo cáo khoa học: The calcium-induced switch in the troponin complex probed by fluorescent mutants of troponin I
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Báo cáo khoa học: The calcium-induced switch in the troponin complex probed by fluorescent mutants of troponin I
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