Báo cáo khoa học: The catalytic significance of the proposed active site residues in Plasmodium falciparum histoaspartic protease
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Alanine mutations of the proposed catalytically essential residues in his-toaspartic protease (HAP) (H34A, S37A and D214A) were generated toinvestigate whether: (a) HAP is a serine protease with a catalytic triad ofHis34, Ser37 and Asp214 [Andreeva N, Bogdanovich P, Kashparov I,Popov M & Stengach M (2004)Proteins55, 705–710]; or (b) HAP is anovel protease with Asp214 acting as both the acid and the base duringsubstrate catalysis with His34 providing critical stabilization [Bjelic S &Aqvist J (2004)Biochemistry43, 14521–14528]....
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Báo cáo khoa học: The catalytic significance of the proposed active site residues in Plasmodium falciparum histoaspartic protease
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Báo cáo khoa học: The catalytic significance of the proposed active site residues in Plasmodium falciparum histoaspartic protease
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