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Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol

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Recombinant glycerol dehydratase ofKlebsiella pneumoniaewas purified to homogeneity. The subunit composition ofthe enzyme was most probablya2b2c2.When(R)- and (S)-propane-1,2-diolswere used independently as substrates, therate with the (R)-enantiomer was 2.5 times faster than thatwith the (S)-isomer. In contrast to diol dehydratase,an iso-functional enzyme,the affinity of the enzyme for the (S)-isomer was essentially the same or only slightly higher thanthat for the (R)-isomer (Km(R)/Km(S) ¼1.5). ...
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Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol

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