Báo cáo khoa học: The effect of HAMP domains on class IIIb adenylyl cyclases from Mycobacterium tuberculosis
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The genesRv1318c, Rv1319c, Rv1320cand Rv3645 1 ofMycobacterium tuberculosisare predicted to code for fourout of 15 adenylyl cyclases in this pathogen. The proteinsconsist of a membrane anchor, a HAMP region and a classIIIb adenylyl cyclase catalytic domain. Expression andpurification of the isolated catalytic domains yielded aden-ylyl cyclase activity for all four recombinant proteins.Expression of the HAMP region fused to the catalyticdomain increased activity in Rv3645 21-fold and slightlyreduced activity in Rv1319c by 70%, demonstrating iso-form-specific effects of the HAMP domains. ...
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Báo cáo khoa học: The effect of HAMP domains on class IIIb adenylyl cyclases from Mycobacterium tuberculosis
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Báo cáo khoa học: The effect of HAMP domains on class IIIb adenylyl cyclases from Mycobacterium tuberculosis
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