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Models for the binding of the sarcin–ricin loop (SRL) of 28Sribosomal RNA to ricinAchain (RTA) suggest that severalsurface exposed arginine residues surrounding the active sitecleft make important interactions with the RNA substrate.The data presented in this study suggest differing roles forthese arginyl residues. Substitution of Arg48 or Arg213withAla lowered the activity of RTA 10-fold. Furthermore,substitutionofArg213withAsp lowered theactivityofRTA100-fold. The crystal structure of thisRTAvariant showed itto have an unaltered tertiary structure, suggesting that thepositively charged state of Arg213 is crucial for activity....
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Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain
