Báo cáo khoa học: The influence of temperature and osmolyte on the catalytic cycle of cytochrome c oxidase
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The influence of temperature on cytochromec oxidase(CCO) catalytic activity was studied in the temperaturerange 240–308 K. Temperatures below 273 K required theinclusion of the osmolyte ethylene glycol. For steady-stateactivity between 278 and 308 K the activation energy was12 kcalÆmol)1; the molecular activity or turnover numberwas 12 s)1at 280K in the absence of ethylene glycol. CCOactivity was studied between 240and 277 K in the presenceof ethylene glycol.
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Báo cáo khoa học: The influence of temperature and osmolyte on the catalytic cycle of cytochrome c oxidase
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Báo cáo khoa học: The influence of temperature and osmolyte on the catalytic cycle of cytochrome c oxidase
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