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The major cellulase secreted by the filamentous fungusTrichoderma reeseiis cellobiohydrolase Cel7A. Its three-dimensional structure has been solved and various mutantenzymes produced. In order to study the potential use ofT. reeseiCel7A in the alkaline pHrange, the thermal sta-bilityofCel7Awas studiedas a functionof pHwith thewild-type and two mutant enzymes using different spectroscopicmethods. Tryptophan fluorescence and CD measurementsof the wild-type enzyme show an optimal thermostabilitybetween pH3.5–5.6 (Tm,62 ± 2C), at which the highestenzymatic activity is alsoobserved, andagradual decrease inthe stability at more alkaline pHvalues. ...
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A
