Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles
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The signature amidase from the extremophile archeumSulfolobus solfatari-cusis an enantioselective enzyme that cleaves S-amides. We report here thatthis enzyme also converts nitriles in the corresponding organic acid, simi-larly to the well characterized amidase from Rhodococcus rhodochrousJ1.The archaeal and rhodococcal enzymes belong to the signature amidasesand contain the typical serine-glycine rich motif. They work at differentoptimal temperature, share a high sequence similarity and both contain anadditional CX3C motif. ...
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Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles
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