Báo cáo khoa học: ThermoFAD, a ThermofluorÒ-adapted flavin ad hoc detection system for protein folding and ligand binding
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In living organisms, genes encoding proteins that contain flavins as a pros-thetic group constitute approximately 2–3% of the total. The fluorescenceof flavin cofactors in these proteins is a property that is widely employedfor biochemical characterisation. Here, we present a modified Thermofluorapproach called ThermoFAD (Thermofluor-adapted flavin ad hoc detec-tion system), which simplifies identification of optimal purification andstorage conditions as well as high-affinity ligands.
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Báo cáo khoa học: ThermoFAD, a ThermofluorÒ-adapted flavin ad hoc detection system for protein folding and ligand binding
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Báo cáo khoa học: ThermoFAD, a ThermofluorÒ-adapted flavin ad hoc detection system for protein folding and ligand binding
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