Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells
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4-Hydroxy-2-trans-nonenal (4-HNE), one of the major endproducts of lipid peroxidation, has been shown to induceapoptosis in a variety of cell lines. It appears to modulatesignalingprocesses inmore thanonewaybecause it has beensuggested to have a role in signaling for differentiation andproliferation. We showfor the first time that incorporationof 4-HNE-metabolizing glutathioneS-transferase (GST)isozyme, hGSTA4-4, into adherent cell lines HLE B-3 andCCL-75, by either cDNA transfection or microinjection ofactive enzyme, leads to their transformation. ...
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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells
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