Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans
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The heterologous expression of tryptophan trytophylquinone (TTQ)-dependent aromatic amine dehydrogenase (AADH) has been achieved inParacoccus denitrificans. The aauBEDAgenes andorf-2from the aromaticamine utilization (aau) gene cluster of Alcaligenes faecaliswere placedunder the regulatory control of themauFpromoter fromP. denitrificansand introduced intoP. denitrificansusing a broad-host-range vector.
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans
Nội dung trích xuất từ tài liệu:
Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans
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