Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation
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In order to determine the amino-terminal sequencerequirements for protein N-myristoylation, site-directedmutagenesis of the N-terminal region was performed usingtumor necrosis factor (TNF) mutants as model substrateproteins. Subsequently, the susceptibility of thesemutants toprotein N-myristoylation was evaluated by metabolic labe-ling in anin vitrotranslation systemusing rabbit reticulocytelysate. A TNF mutant having the sequence MGAAAAAAAA at its N-terminus was used as the starting sequenceto identify elements critical for protein N-myristoylation....
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation
Nội dung trích xuất từ tài liệu:
Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation
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