Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant
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Conservedphenylalanine 35 is one of the hydrophobic patchresidues on the surface of cytochromeb5(cytb5). This patchis partially exposed on the surface of cyt b5while its buriedface is in direct van der Waals’contact with heme b. Resi-dues Phe35 and Phe/Tyr74 also form an aromatic channelwith His39, which is one of the axial ligands of heme b. Bysite-directedmutagenesiswehaveproduced threemutantsofcytb5:Phe35fiTyr, Phe35fiLeu, and Phe35fiHis.
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Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant
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Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant
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