Báo cáo lâm nghiệp: Apoplast: a sensitive site for assessing some biochemical in Norway spruce needles

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Apoplast: a sensitive site for assessing some biochemical in Norway spruce needles...
Nội dung trích xuất từ tài liệu:
Báo cáo lâm nghiệp: "Apoplast: a sensitive site for assessing some biochemical in Norway spruce needles"Apoplast: a sensitive site effects offor assessing some biochemical 32 O or SOin Norway spruce needles H.G. F.J. Castillo Greppin Ogier, Biochemistry and Physiology, University of Geneva, CH-1211, Geneva 4,Laboratory of PlantSwitzerland (IWF) and in the residual cell materialIntroduction (RCM). The plants treated in summer remained 12 wk longer in the chambers inThe study of the cell wall-plasma mem- order to assess any visible injury causedbrane interphase is of great importance for by 0 in autumn. 3the understanding of gaseous air pollu-tants and leaf cell interactions. In the apo-plast liquid phase, the pollutants are solu-bilized and they can generate oxidative Materials and Methodsproducts (Tingey and Taylor, 1982). Forexample, 0 or S0 could lead to H 32 0 2 Two groups of 20 clone saplings (4 yr old graft-production (Tingey and Taylor, 1982; Khan ed P. abies) were selected from the nursery ofand Malhotra, 1982). In order to protect the Swiss FedEaral Institute of Forestry Re-the plasma membrane and the compo- search (Birmensdorf, CH), one group for each experiment. Prior to fumigation, the plants werenents of the extracellular matrix, cells are distributed randomly into 4 semi-open topbelieved to dispose of oxidant-scavenging chambers (5 individuals per chamber). Currentmechanisms. One of the enzymatic sys- year old needles and 1 yr old needles weretems which could play a protective role analyzed in samples harvested at the end of theagainst oxidative stresses includes per- fumigation period. The experimental approach is shown in Table I.oxidases (Castillo and Greppin, 1988). The IWF was obtained after infiltration of Peroxidase activity, with guaiacol as the buffer (40 mM, pH 4.5), 0.1 M KCI, phosphateelectron donor, and protein content were 3 pM EDTA, and centrifugation (10 000 x g,measured in Norway spruce needles 4°C, 10 min) according to Castillo et al. (1987). The RCM extract was obtained from 0.5 g of(Picea abies (L.) Karst) after fumigation the remaining needles, which were ground(24 h/d) in semi-open top chambers, for under liquid nitrogen, in the presence of PVP12 wk in summer with 0 or for 10 wk in 3 (0.5 g), then solubilized with 3 ml of phosphatewinter with S0 These parameters were . 2 buffer (66 mWl, pH 7), and centrifuged 10 (10 000 x g, 4°C, min).followed in the intercellular washing fluid Peroxidase activity was assayed by mea- This enzyme activity was respectively).suring the oxidation of guaiacol at 470 nm. not affected in the IWF by S0 treatment. 2This activity was carried out using phosphate The only noticeable change in the RCMbuffer (66 mM, pH 6.1), 16 mM guaiacol, increase in 1 yr old needles of the was an3.3 mM H and 0-10 ul of enzyme extract. 0 2 ambient air-treated plants (124%Protein contents were determined according to summerBradford (1976) using a Bi ...