Báo cáo lâm nghiệp: In vitro propagation of Prosopis P. cineraria and P. juliflora) species

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: In vitro propagation of Prosopis P. cineraria and P. juliflora) species...
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Báo cáo lâm nghiệp: "In vitro propagation of Prosopis P. cineraria and P. juliflora) species"In vitro propagation of Prosopis species (P. chilensis,P. cineraria and P. juliflora)C.A. Batchelor D. Yao, M.J. Koehler P.J.C. HarrisDepartment of Biological Sciences, Coventry Polytechnic, Priory Street, Coventry CV1 5FB, U.K. immersed in an anti-oxidant solution (100 mg/IIntroduction citric acid, 50 mg/I ascorbic acid, 100 mg/I poly- vinyl pyrrolidone) for 15 min. Murashige and Skoog medium was used with 8 g/I agar, 30 g/IThe genus Prosopis has attracted con- sucrose, 1.6 g/l glutamine and 81 combinationssiderable interest for forestry in arid areas. of plant hormones, including kinetin (K)although normally propagated by seed, (0.05-15 mgA), benzylamino purine (BA)vegetative propagation of selected Proso- (0.05-15 mg/I), indole acetic acid (IAA) (1-10 mg/I), indole butyric acid (IBA)pis plants from variable populations and (1-15 mg/I) and naphthalene acetic acid (NAA)from natural hybrids may also be desir- (1-15 mg/I). Between 4 and 16 explants wereable. Propagation by cuttings has been placed on each medium and incubated at 25°Creported (e.g., Felker and Clark, 1981). ). with a 16 h pho’toperiod and a photon flux den-The use of tissue culture techniques to sity of 65-200 !E/m2 for 51 d.regenerate plants from nodal explants hasalso been reported for P. cineraria (Goyaland Arya, 1984), P. tamarugo and P. Resultschilensis (Jordan and Balbao, 1985), andP, alba (Tabone et al., 1986). In this study,tissue culture media were evaluated for Without hormones, results for rooting per-root initiation, shoot proliferation and shoot centage, mean number of shoots/explantgrowth of P. chilensis, P. cineraria and P. node and mean number of nodes/regen-juliflora. erated shoot were, P. chilensis, 0%, 0.8, 1.2, P. cineraria, 13%, 0.8, 1.0, and P. juli- flora, 6%, 0.4, 1.0. A summary of the most successful hormone treatments is given inMaterials and Methods Table I. High levels of BA induced shoot proliferation of P. chilensis with 15 mg/lExplants with 1 or 2 nodes were taken from the BA, 5 mg/l NAA giving the highest mean 6 nodes of the main stem andyoungest of 4.3 shoots/node. Similar levels of Kbranches of 3-12 mo old, greenhouse-grown were much less effective for shoot prolif-stock plants. Explants were surface sterilized in eration. Shoot growth of P. chilensis was70% industrial methylated spirit for 1 min and greatest (5 nodes/shoot) with 0.05 mg/I K,5% (v/v) sodium hypochlorite for 5-10 min, and IBA. 0.05 mg/I BA with 3 mg/I IBA, the greatest (75%) percentage of rooted3 mg/I mg/I K, with 3 or 15 mg/I IBA 1 0.05 explants.or orinduced rooting. 0.05 mg/I K, 3 mg/I IBA Shoot proliferation of P. cineraria was the number (2.5) of also promoted by high levels of BA, greatestgave and 1 mg/I IBA 10 mg/I BA, 5 mg/I NAA resulting in 3 mg/I K, 15roots/explantshoots/node. K failed to induce multiple trations of BA, but not of K in combinationshoot production in P. cineraria. Shoot with auxins, promote shoot proliferation probably by stimulating axillary budgrowth was greatest (3 nodes/shoot) with3 mg/I K, 23 mg/l NAA but 1 mg/I BA, growth. Medium to high concentrations of3 mg/I IBA gave reduced leaf abscission. auxin in combination with low t ...