Báo cáo lâm nghiệp: Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase

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Báo cáo lâm nghiệp: "Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase"Sexual reproduction in Populus I. Some physiologicaland biochemical events of the progamic phase C. Dumas 2 2 GagetM. Villar M. 1INRA,1 Station D’Amélioration des Arbres Forestiers, Ardon, F-45160 Olivet, and dAm6liorationLaboratoire2 de Reconnaissance Cellulaire et Am6lioration des Plantes, INRA 23879, UniversitéLyon I, 43, bd 11-Novembre-1918, F-69622 Villeurbanne Cedex, France tions, silver nitrate staining and homogenates ofIntroduction pollinated stigma performed according to were the procedures ot’ Villar et al. (1988). Each of the 2 crosses was represented by 3 series ofHybridization in Populus breeding pro- stigmatic extract!., in accordance with thegrams is limited by sexual incompatibility growth kinetics (0.6 and 20 h after pollination).barriers, whose cellular and molecular Protein patterns of these extracts were com-mechanisms are not yet known. In an pared on a single gel. Glycoprotein revelation (concanavalin A-binding proteins) on IEF poly-attempt to understand the nature of inter- acrylamide gel was adapted from Hawkesspecific incompatibility in Populus, we (1982). j3-Ga!actosidase visualization was op-have explored the interactions between timized from the protocol of Singh and Knoxmale and female partners (pollen-pistil) in (1985).compatible and incompatible crosses P.nigra (female) x P. nigra (male) and P.nigra (female) x P. alba (male) (Villar, Results and Dis;cussion1987). Kinetics of pollen tube growth (visualiza- tion using the AI3F method) have demon-Materials and Methods strated distinct behaviors of P nigra and P alba pollen tubes inside P nigra pistils (Fig. 1P alba pollen tubes exhibit aP. nigra and P. alba branches were obtained unique S-shaped growth curve and anfrom the INRA Forestry station in Or[6ans(France). Pollen was collected and stored in arrested growth near the stylodium. Onclosed vials at -18°C. Kinetics of pollen tube the other hand, P nigra pollen tubes ex-growth in vivo were studied in growth chambers hibit 2 growth phases, respectively, in the(20°C). Pollen tubes in the stylar tissues were stigmatic tissues and in the ovarian cavity.visualized using the aniline blue fluorescence P, nigra and P alba curves diverge 5 hmethod (ABF method, Dumas and Knox, 1983). after pollination (20°C), corresponding Isoelectric focusing (IEF) of pollinic and stig-matic proteins, gel preparation, focusing condi- precisely to the deposition of the first observed, Differences plug inside pollen tubes. Thiscallose glycoproteins. were distinct type of to thedivergence could likely be related to a according cross: one in the compat- increaseschange in the physiology of the compat- only glycoprotein ible cross. Moreover, f3-Galactosidase ac-ible pollen tube, shifting from an auto- ...