Báo cáo nghiên cứu khoa học: Thành phần hoá học của tinh dầu quả loài giổi (Michelia sp.) ở Việt Nam - một nguồn cung cấp safrol

Tuyển tập các báo cáo nghiên cứu khoa học hay nhất của trường đại học vinh năm 2008 tác giả: 5. Hà Thị Thanh Hải, Chu Thị Thanh Lâm, Trần Đình Thắng, Nguyễn Xuân Dũng, Thành phần hoá học của tinh dầu quả loài giổi (Michelia sp.) ở Việt Nam - một nguồn cung cấp safrol.
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Báo cáo nghiên cứu khoa học: "Thành phần hoá học của tinh dầu quả loài giổi (Michelia sp.) ở Việt Nam - một nguồn cung cấp safrol"H.T.T.Hai, C.T.T. Lam, T.D.Thang, N.X.Dung Chemical composition ..., TR. 42-46 Chemical composition of the fruit oil of Michelia sp. from Vietnam - A New Natural Source of Safrole Ha Thi Thanh Hai (a), Chu Thi Thanh Lam (a), Tran Dinh Thang , Nguyen Xuan Dung (b) (c) Abstract. The volatile components of the fruit oil of Michelia sp. from Nghean province, were analysed by Capillary Gas chromatograph (GC) and Gas chromatograph/Mass spectroscopy (GC/MS) as well as by background subtraction of coeluting compounds. A total 33 compounds were identified of which the major component was safrole (93.41%). The lesser amount were limonene (1.19%) and linalool (2.46%). This result shows that Michelia sp. from Nghe an as a new source of safrole. 1. Introduction The Magnoliaceae contains about 15 genera and ca. 300 species most of thespecies occur primarily in East and South-East Asia but also in South-East NorthAmerica, and tropical America. There are 50 species of the genus Micheliadistributed in tropical and subtropical Asia [1]. Michelia species are commonly cultivated for their fragrant flowers and asornamentals. The flowers of some Michelia are marketed for their scent, used inmaking garlands, placed between stored clothes, sprinkled in bridal beds and usedin the preparation of scented hair lotions. In Thailand an infusion of the flowers isapplied as a cosmetic after bathing. In Indonesia an infusion made by steeping thebitter bark in water is given against fever. Several Michelia species, yield a timberof minor economic importance, used for light construction, vehicle bodies, packingcases and for the production of veneer plywood, wood-wood board and as fuel [2]. M. balansae (A.DC.) Dandy is an ornamental tree planted in gardens andgrowing wild. The flower is beautiful and fragrant and can be used for the extractionof essential oil. Only one report on flower oil components of this species. Chemicalconstituents of the flower oil of M. balansae (A.DC.) Dandy from South ChinaBotanical Garden, Guangzhou are as follows: butyl acetate (2.18%), ethyl butyrate(0.23%), 1- ethoxyethyl acetate (0.60%), ethyl 2-methylbutyrate (0.43%), 2-methylbutyl acetate (2.80%), 2-metylpropyl 2-methylpropanoate (1.86%), methylhexanoate (0.21%), α-pinene (0.12%), 1-metylpropyl butyrate (0.34%), β -pinene(0.10%), β -myrcene (0.10%), ethyl hexanoate (61.01%), 2-methylpropyl 3-butyrate(0.17), 2-methylbutyl 2-metyl 2-metylpropanote (0.10%), p-cymene (0.42%), limonene(15.70%) ethyl 2-hexenoate (0.10%), camphor (0.27%), 2-methylpropylhexanoate(1.32%), and carvone (0.10%) [3]. The volatile components of the leaf oil of M. balansae (A.DC.) Dandy from theNghean province, Vietnam were analyzed by Capillary GC, GC/MS as well as bybackground substraction of coeluting compounds. The major components in the leaf.NhËn bµi ngµy 29/5/2008. Söa ch÷a xong 28/10/2008.42tr−êng §¹i häc Vinh T¹p chÝ khoa häc, tËp XXXVII, sè 4a-2008oil were α-pinene (18.41%), α-, β -phellandrene (17.36% and 7.42%), germacrene D(17.98%) and bicyclogermacrene (7.55%). The lesser amounts were β -myrcene(3.88%), δ-elemene (5.11%) and β -caryophyllene (3.47%) [4]. The leaf oil of M. montana from India were prepared by hydrodistillation andanalysed by a combination of GC and GC/MS. Thirty components have beenidentified. The major constituents were found to be asaricin (81.8%) and safrole(13.0%) [5]. The essential oil in Michelia leaves was extracted by steam distillation. Theoil obtained was dried with anhydrous magnesium sulfate. Thirty three peaks wereseparated by GC, and 27 of them were identified by MS with NBS mass spectraldata and Wiley/NBS registry of mass spectral data. The structure of linalool wasfurther verified by GC/FTIR. The identified constituents represent 97% of the peakarea of the essential oil on FID. The major chemical constituent of them are linalool,trans-caryophyllene and diethyl-o-phthalate [6]. In the course of the systematic study of Michelia from Indochina, mono-,sesquiterpenes and other components of the fruit oil of Michelia sp. from Ngheanprovince have been investigated. 2. Experimental Source: Fruits of Michelia sp. were collected in Pu Huong National Park,Nghean province. A voucher specimen (NLN5) is deposited at the Herbarium of theInstitute of Ecology and Biological Resources, Vietname Academy of Science andTechnology. Dried fruits were shredded and their oil were obtained by steam distillationfor 3h at normal pressure, according ...