Báo cáo Y học: A comparison of the urea-induced unfolding of apo¯avodoxin and ¯avodoxin from Desulfovibrio vulgaris
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The kinetics and thermodynamics of the urea-inducedunfolding of ¯avodoxin and apo¯avodoxin fromDesulfo-vibrio vulgariswere investigated by measuring changes in¯avin and protein ¯uorescence. The reaction of urea with¯avodoxin is up to 5000 times slower than the reaction withthe apoprotein (0.67 s)1in 3 Murea in 25 mMsodiumphosphate at 25°C), and it results in the dissociation ofFMN.
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: A comparison of the urea-induced unfolding of apo¯avodoxin and ¯avodoxin from Desulfovibrio vulgaris
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: A comparison of the urea-induced unfolding of apo¯avodoxin and ¯avodoxin from Desulfovibrio vulgaris
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