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Equilibrium studies on the acid included denaturation ofstem bromelain (EC 3.4.22.32) were performed by CDspectroscopy, ¯uorescence emission spectroscopy andbinding of the hydrophobic dye, 1-anilino 8-naphthalenesulfonic acid (ANS). At pH 2.0, stembromelain lacks awellde®ned tertiary structure as seen by ¯uorescence and near-UVCDspectra.Far-UVCDspectra showretentionof somenative like secondary structure at pH 2.0. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, IndiaEquilibrium studies on the acid included denaturation of stem bromelain (EC 3.4.22.32) were performed by CD spectroscopy, ¯uorescence emission spectroscopy and binding of the hydrophobic dye, 1-anilino 8-naphthalene sulfonic acid (ANS). At pH 2.0, stem bromelain lacks a well de®ned tertiary structure as seen by...
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Báo cáo Y học: Characterization of a partially folded intermediate of stem bromelain at low pH
