Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes
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A nitroalkane-oxidizing enzyme gene (naoA) was clonedfrom a genomic DNA library ofStreptomyces ansochromo-genes7100. The deduced protein (NaoA) of this gene con-tains 363 amino acids and has high similarity to severalnitroalkane-oxidizing enzymes from various micro-organ-isms.ThenaoAgenewas subcloned intoanexpressionvectorpET23b and overexpressed inEscherichia coliBL21(DE3).The protein was then purified, and its characteristics werestudied. Experimental results showed that NaoA can con-vert 1-nitropropane, 2-nitropropane and nitroethane intothe corresponding carbonyl compounds....
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes
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