Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization
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Random PCR mutagenesis was applied to theThermusthermophilus xylA gene encoding xylose isomerase. Threecold-adapted mutants were isolated with the followingamino-acid substitutions: E372G, V379A (M-1021),E372G, F163L (M-1024) and E372G (M-1026). The wild-type and mutated xylAgenes were cloned and expressedin Escherichia coliHB101 using the vector pGEMÒ-TEasy, and their physicochemical and catalytic propertieswere determined.
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Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis Gene cloning and protein characterization
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