Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii
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Mammalian tubulins and actins attain their native con-formation following interactions with CCT (the cytosolicchaperonin containing t-complex polypeptide 1). To studythe b-tubulin folding in lower eukaryotes, an isotype ofb-tubulin (b-T1) from the Antarctic ciliate Euplotesfocardii, was expressed in Escherichia coli. Folding analysiswas performed by incubation of the35S-labeled, denaturedb-T1 in the presence, or absence, of purified rabbit CCTand cofactor A, a polypeptide that stabilizes foldedmonomericb-tubulin. ...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii
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