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AnN-acyl-D-amino acid amidohydrolase (N-D-AAase) wasidentified in cell extracts of a strain, Iso1, isolated from anenvironment containing N-acetyl-D-methionine. The bac-terium was classified as Variovorax paradoxusby phylo-genetic analysis. The gene was cloned and sequenced. Thegene consisted of a 1467-bpORF encoding a polypeptide of488 amino acids. TheV. paradoxus N-D-AAase showedsignificant amino acid similarity to theN-acyl-D-amino acidamidohydrolases of the two eubacteriaAlcaligenes xylo-soxydans A-6 (44–56% identity),Alcaligenes facelisDA1(54% identity) and the hyperthermophilic archaeon Pyro-coccus abyssi(42% identity)....
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production
