Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
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Two secreted acid phosphatases (SAP1 and SAP2) weremarkedly up-regulated during Pi-starvation of tomatosuspension cells.SAP1 and SAP2 were resolved during cat-ion-exchange FPLC of culture media proteins from 8-day-old Pi-starved cells, and purified to homogeneity and finalp-nitrophenylphosphatehydrolyzing specificactivitiesof 246and 940lmol Pi producedÆmin)1mgÆprotein)1, respect-ively.SDS/PAGE, periodic acid-Schiff staining and analyt-ical gel filtration demonstrated that SAP1 and SAP2,respectively, exist as 84 and57 kDa glycosylatedmonomers....
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
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