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Báo cáo Y học: Reconstructing the replication complex of AcMNPV

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Baculoviruses are well known for their large, circular, dou-ble-stranded DNA genomes. The type member, AcMNPV,is the best characterized andundergoes a successionof early,late and very late gene expression during its infection cycle.The viral genes involved inDNAreplicationhave previouslybeen identified and their products are required for the acti-vation of late gene expression. In this study, we FLAG- andHA-tagged the replication late expression factors ofAcMNPV, examined their expression and functionalactivities by CAT assay and Western blot analysis, anddetermined their subcellular localization in transfectedcells by subcellular fractionation and immunofluorescentmicroscopy....
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Báo cáo Y học: Reconstructing the replication complex of AcMNPV

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