Báo cáo Y học: The presence of phosphate at a catalytic site suppresses the formation of the MgADP-inhibited form of F1-ATPase
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F1-ATPase is inactivated by entrapment of MgADP incatalytic sites and reactivatedbyMgATPorPi.Here, usingamutant a3b3c complex of thermophilic F1-ATPase(aW463F/bY341W) and monitoring nucleotide binding by¯uorescence quenching of an introduced tryptophan, wefound that Pi interfered with the binding of MgATP toF1-ATPase, but binding ofMgADPwas interferedwith to alesser extent.
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Báo cáo Y học: The presence of phosphate at a catalytic site suppresses the formation of the MgADP-inhibited form of F1-ATPase
Nội dung trích xuất từ tài liệu:
Báo cáo Y học: The presence of phosphate at a catalytic site suppresses the formation of the MgADP-inhibited form of F1-ATPase
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Report medicine traditional medicine scientific reports Structural biochemistry oxidation bacteriaTài liệu liên quan:
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