Biological activity of methanolic extract derived from Ipomoea-pes-caprae (L.) collected in Xuan Thuy national park

In this study, methanolic extracts from the stems, leaves and flowers of Ipomoea pes-caprae that were collected in Xuan Thuy National Park were evaluated for their antibacterial and antioxidative activities, and their ability to inhibit plant growth. The DPPH free radical scavenging activity of crude methanol extracts was evaluated.
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Biological activity of methanolic extract derived from Ipomoea-pes-caprae (L.) collected in Xuan Thuy national park JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci., 2013, Vol. 58, No. 9, pp. 139-145 This paper is available online at http://stdb.hnue.edu.vn BIOLOGICAL ACTIVITY OF METHANOLIC EXTRACT DERIVEDFROM Ipomoea pes-caprae (L.) COLLECTED IN XUAN THUY NATIONAL PARK Dao Thi Sen, Dao Van Tan and Hoang Thi La Faculty of Biology, Hanoi National University of Education Abstract. Ipomoea pes-caprae (L.) Sweet (Convolvulaceae), a common species in Xuan Thuy National Park, Nam Dinh Province, is thought to be a potential source of bioactive compounds. In this study, methanolic extracts from the stems, leaves and flowers of Ipomoea pes-caprae that were collected in Xuan Thuy National Park were evaluated for their antibacterial and antioxidative activities, and their ability to inhibit plant growth. The DPPH free radical scavenging activity of crude methanol extracts was evaluated. The extract of flower had the highest radical scavenging activity (IC50 = 0.33 mg/mL) while stem extract showed the lowest activity (IC50 = 3.39 mg/mL). Antibacterial activity was tested using the agar diffusion method and it was found that the extracts showed antibacterial activity against Bacillus subtilis and Vibrio parahaemolyticus. The stem extract showed the highest activity against V. parahaemolyticus (D, 14.7 mm) and was the only extract that inhibited E. coli growth. However, no extract had an inhibitory effect on Vibrio spp. or Staphyloccus aureus. Extracts of all parts of the plant inhibited the growth of the roots of Raphanus salivus seedlings 44 - 73%. Keywords: Ipomoea pes-caprae (L.) Sweet, DPPH, Antibacterial activity, bioactivity.1. Introduction Plants have been used as an important source of medicine for thousands of years.Medicinal plants are the most important source of life saving drugs for the majority ofthe world’s population. Recently, several distinct chemicals derived from plants have beenused as a component in important drugs. The World Health Organization (WHO) hasestimated that up to 80% of the human population has used traditional herbal remedies asmedicine [12]. The genus Ipomoea (Convolvulaceae) consists of over 200 species which are widelydistributed in tropical and subtropical countries. Some of them are used in folk medicine toReceived May 24, 2013. Accepted November 26, 2013.Contact Dao Thi Sen, e-mail address: daosenk53sinh@yahoo.com 139 Dao Thi Sen, Dao Van Tan and Hoang Thi Latreat diseases [6,13]. Ipomoea pes-caprae grows profusely in low-lying broad coastal sanddunes. It is known to inhibit sand erosion and it thrives in a marine environment of highsalinity, high temperature, continuous exposure to the sun, incessantly tidal fluctuation,intense rain and wind, etc. In Vietnam, I. pes-caprae is a commonly used traditional medicine. Dried leavesand stems are used to treat stomach pain, fever and rheumatoid arthritis. This planthas also been used to treat inflammation, skin disease, burn, wounds and ulcers [7]. I.pes-caprae has been used as a herbal remedy in other countries to treat inflammations,colic, diuretic disorders and gonorrhoea [8]. Preclinical and clinical investigations haveidentified pharmacological properties of this plant [11]. It has been reported that I.pes-caprae inhibited the contraction of guinea pig ileum relative to its concentration.The same extract also showed considerable anti-inflammatory action when evaluatingin different experimental models [11]. In Vietnam, recent studies on I. pes-caprae focused primarily on identification andecological characterization. Therefore, the determination of antioxidant and antibacterialactivity, and inhibition of growth is necessary to characterize pharmacological propertiesand possibly lead to future applications. In this study, the methanolic extract from variousparts including roots, stems, leaves and flowers of Ipomoea pes-caprae that were collectedin Xuan Thuy National Park, Nam Dinh Province, were evaluated for their antibacterialand antioxidative activities and possible ability to inhibit plant growth.2. Content2.1. Material and methods * Plant materials: Fresh stems, leaves, flowers of I. pes-caprae, free from disease,were collected in Xuan Thuy National Park, Nam Dinh Province, in September, 2012,and they were washed thoroughly 2 - 3 times under running tap water, shade-dried andpowdered before being used for extraction. * Preparation of methanolic extract: Shade-dried powder of I. pes-caprae wassoaked in methanol f ...