Optimization of E. coli culture conditions for efficient DNA uptake by electroporation

Bacterial transformation is a significant breakthrough in the field of molecular biology for cloning purposes. A variety of chemical and physical methods are utilized for enhancing bacterial competence. The present study was designed, via the consolidationof chemical and physical methods, to improve competence of cells for high transformation efficiency up to 109 transformants/µg of DNA.
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Optimization of E. coli culture conditions for efficient DNA uptake by electroporation