Structure and cytotoxic activity of a sulfated polysaccharide from green seaweed Caulerpa racemose collected on Nha Trang bay, Viet Nam

In this work, a new sulfated polysaccharide was isolated from green seaweed Caulerpa racemosa harvested at Nha Trang sea. The structure of obtained polysaccharide was determined and its cytotoxic activity was evaluated. The results showed that structure of the polysaccharide has a main chain of (1→4)-β-D-galactopyranose residues and branches are (1→3)-α-L-glucopyranose-2,4-disulfate residues linked to the main chain at C-6 position.
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Structure and cytotoxic activity of a sulfated polysaccharide from green seaweed Caulerpa racemose collected on Nha Trang bay, Viet NamCite this paper: Vietnam J. Chem., 2023, 61(S3), 59-64 Research ArticleDOI: 10.1002/vjch.202300058Structure and cytotoxic activity of a sulfated polysaccharide from green seaweed Caulerpa racemose collected on Nha Trang bay, Viet Nam Quach Thi Minh Thu1, Dang Vu Luong1, Nguyen Quang Tam1, Nguyen Thi Nu2, Le Thi Hong Nhung3, Thanh Thi Thu Thuy1* 1 Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 10000, Viet Nam 2 Hanoi Metropolitan University, 98 Duong Quang Ham, Cau Giay, Hanoi 10000, Viet Nam 3 Hanoi University of Industry, 298D, Cau Dien, Nam Tu Liem, Hanoi 10000, Viet Nam Submitted February 18, 2023; Revised April 24, 2023; Accepted May 19, 2023Abstract In this work, a new sulfated polysaccharide was isolated from green seaweed Caulerpa racemosa harvested at NhaTrang sea. The structure of obtained polysaccharide was determined and its cytotoxic activity was evaluated. The resultsshowed that structure of the polysaccharide has a main chain of (1→4)-β-D-galactopyranose residues and branches are(1→3)-α-L-glucopyranose-2,4-disulfate residues linked to the main chain at C-6 position. The sulfated polysaccharideshowed cytotoxic activity against three cancer cell lines Hela (cervical cancer) HT29 (human colorectal adenocarcinoma),and 8505c (human undifferentiated thyroid carcinomas) with IC50 values of 57.52±2.08, 51.16±1.33 and 45.68±1.78µg/mL, respectively Keywords. Sulfated polysaccharide, green seaweed, Caulerpa racemosa, structure, cytotoxic activity.1. INTRODUCTION exhibited inhibition effect on K562 cells in both in vitro and in vivo; Cavas et al.[8] investigated theGreen seaweeds belonging to the Chlorophyta antiproliferative and apoptotic effects of sulfateddivision are found in both marine and freshwater. polysaccharide from C. racemosa collected atThey are rich sources of sulfated polysaccharides Mediterranean Sea. A sulfated polysaccharidewith anticoagulant activity,[1] cytotoxic activity,[2] contained galactose, glucose, arabinose and xyloseantioxidant activity, etc.[3] was extracted from C. racemosa, it has great anti- Caulerpa is a green seaweed genus in the family herpetic activity.[9] The reported results indicated theCaulerpaceae. It is widely distributed in tropical and structural complexity and wildly bioactive function ofsubtropical areas. Sulfated polysaccharides extracted C. racemosa polysaccharides.from Caulerpa genus were reported so far. Rao et al. In our previous works,[10,11] two sulfatedstudied the structural features of a sulfated polysaccharides were extracted from green seaweedspolysaccharide from Caulerpa taxifolia the results Ulva lactuca and Chaetomorpha linum collected atshown that the polysaccharide has a very complex Nha Trang bay, Vietnam. They were identified asstructure.[4] It was indicated that polysaccharide from ulvan and arabinogalactan, respectively, and showedCaulerpa lentillifera having anti-diabetic activity.[5] great cytotoxic activity against several human cancerIn addition, polysaccharide from Caulerpa cell lines.sertularioides was reported to have antioxidant, In Vietnam, Caulerpa racemosa (“Rong guộtantibacterial, and immunological activities.[6] chùm” or “Rong Cầu lục chùm” in Vietnamese) was Among Caulerpa species, Caulerpa racemosa, a used as a traditional medicine in treatment of highlarge edible green alga, was attracted more attention blood pressure disease. However, to the best of ourof scientist because of its complex structure and knowledge, there is no study on the polysaccharideuseful bioactive function. Ji and Zhao[7] analyzed the from C. racemosa harvested in Vietnam sea has beenchemical constituents of polysaccharide isolated from reported. The aims of the present work are to isolateC. racemosa and reported that the polysaccharide sulfated polysaccharide from the green seaweed C.contained two main sugars, glucose and galactose and racemosa, to determine its structure, and to59 Wiley Online Library © 2023 Vietnam Academy of Science and Technology, Hanoi & Wiley-VCH GmbH ...