Summary of Biotechnology doctoral thesis: Study on development of DNA barcodes and research on in vitro micropropagation of Paramignya trimera

Thesis objectives: Evaluating the distribution of morphological characteristics, building a distribution map of the population of P. trimera and some species of the genus Paramignya; determining the genetic relationship of the P. trimera population by SSR markers; identification of DNA barcodes for identification of P. trimera; developed the protocol of in vitro micropropagation of P. trimera collected in Khanh Hoa, Vietnam.
Nội dung trích xuất từ tài liệu:
Summary of Biotechnology doctoral thesis: Study on development of DNA barcodes and research on in vitro micropropagation of Paramignya trimeraMINISTRY OF EDUCATION AND VIETNAM ACADEMY TRAINING OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY ----------------------------- Phi Thi Cam MienStudy on development of DNA barcodes and research on in vitro micropropagation of Paramignya trimera Specialized: Biotechnology Code number: 9.42.02.01 SUMMARY OF BIOTECHNOLOGY DOCTORAL THESIS Ha Noi, 2021 The thesis was completed at: Department Graduate University Science and Technology, Institute of Biotechnology Supervisor: Assoc.Prof. Dr Chu Hoang Ha Reviewer 1: … Reviewer 2: … Reviewer 3: ….The thesis will be defended at:..................................................................................................................................................................................................................................................................................Time: ..............................................................................................................................The thesis could be found at: National Library of VietNam Library of Graduate University Science and Technology Institute of Biotechnology 1 INTRODUCTION1. Introduction The Paramignya trimera (Oliv.) Guill belonging to the family Rutaceae, distributedmainly in Khanh Hoa and Ninh Thuan provinces. According to published documents, Vietnamhad 7 species of the genus Paramignya. P. trimera is a small woody, creeping, wild tree,distributed in areas over 200 meters, where has arid climate, thin topsoil. It has been widelyused in traditional and folk medicine as an herbal plant for treating the liver diseases, bloodpressure and cancer. Recently, many studies have shown that the root of P. trimera containsmany alkaloids, saponins, courmarins and triterpenoids, which have effects on acute hepatitisand inhibited some cancer cell lines. In particular, P.trimera extract is so safe for use. Because of the highly effects of the P. trimera root for treating some cancer cell lines,the price of this root is very high, so its over exploited exhaustion. In addition, due to the highability of cross-pollination and highly frequency mutations, combined with the phenomenonof asexual reproduction and polyploidy, the phenotype of P.trimera in nature is very diverse,with many similar characteristics with other plants in the genus Paramignya. The existence ofsuch hybrids makes them difficult to distinguish and confusing based on only morphology. Itsexistence of many types of P.trimera such as outcrosses between closely related species of thegenus Paramignya, so it is difficult to identify accurately to store, preserve and multipropagation to develop P.trimera area. Therefore, it is necessary to accurately identify theP.trimera species for future research, and developing the medicinal plant areas to multipropagation. So, the study Study on development of DNA barcodes for identification and invitro micropropagation of P. trimera to accurately identify P. trimera species and propagatethis species in order to conserve and effectively exploit this species of high medicinal value.2. Thesis objectives - Evaluating the distribution of morphological characteristics, building a distribution mapof the population of P. trimera and some species of the genus Paramignya - Determining the genetic relationship of the P. trimera population by SSR markers - Identification of DNA barcodes for identification of P. trimera - Developed the protocol of in vitro micropropagation of P. trimera collected in KhanhHoa, Vietnam3. Thesis contents - Investigate, collect and build a map of the distribution of the P.trimera population - Evaluation of the genetic diversity of the population of P.trimera and somespecies of the genus Paramignya by SSR - Research on building the DNA barcodes for identification of the P.trimera - Study an in vitro micropropagation to conservation of P.trimera species4. Thesis contributions - Additional scientific data to identify the morphological characteristics and taxonomy ofP.trimera in Khanh Hoa province - A database of DNA sequences for the P.trimera has been built and registered in NCBI,and initially developed DNA markers to make a foundation for the development of DNAbarcodes. - - Contributing to creating a scientific ba ...