Summary of Biotechnology doctoral thesis: Study to produce oligochitosan-silica nano and investigate the induced systemic resistance against anthracnose disease caused by Colletotrichum spp. on hot chilli (Capsicum frutescens L.)

The objectives of the thesis: Identify the pathogenicity potential and classificate at species level of Colletotrichum spp. isolated from hot chilli in Vietnam; produce stable oligochitosan-silica nano, which potentially highly induced effectively the systemic resistance against anthranose disease on hot chilli, based on the combination of low molecular weight chitosan with nano silica.
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Summary of Biotechnology doctoral thesis: Study to produce oligochitosan-silica nano and investigate the induced systemic resistance against anthracnose disease caused by Colletotrichum spp. on hot chilli (Capsicum frutescens L.) MINISTRY OF VIETNAM ACADEMY EDUCATION AND OF SCIENCE AND TRAINING TECHNOLOGYGRADUATE UNIVERSITY SCIENCE AND TECHNOLOGY ---------------------------- PHAM DINH DUNGStudy to produce oligochitosan-silica nano and investigate the induced systemic resistance against anthracnose disease caused by Colletotrichum spp. on hot chilli (Capsicum frutescens L.) Major: Biotechnology Code: 9 42 02 01 SUMMARY OF BIOTECHNOLOGY DOCTORAL THESIS Ho Chi Minh City – 2020The thesis was completed at: Graduate University of Scienceand Technology - Vietnam Academy of Science andTechnologySupervisor 1: Assoc. Prof., Dr. NGUYEN TIEN THANGSupervisor 2: Assoc. Prof., Dr. BUI VAN LEReviewer 1:Reviewer 2:Reviewer 3:The thesis shall be defended in front of the Thesis Committeeat Academy Level at Graduate University of Science andTechnology - Vietnam Academy of Science and TechnologyAt...... hour....... date...... month...... year 20..The thesis can be found at:- The National Library- The Library of Graduate University of Science andTechnology 1 INTRODUCTION 1. The necessity of the thesis Hot chili (Capsicum sp.) is a spice plant grown in thetropics and consumed around the world due to its higheconomic value. However, diseases caused by fungi, virusesand bacteria are the major constraints to hot chili production.Among the diseases on hot chilli, pathogenic fungus is one ofthe main reasons cause of loss 10-80% hot chilli production inVietnam, India, Thailand, Korea… Common diseases on hotchili are caused by Rhizoctonia solani, Colletotrichum spp.,Botrytis cinerea, Fusarium oxysporum, Phythopthoracapsica,…, anthracnose disease, caused by Colletotrichum spp.is one the most destructive diseases restricting hot chiliproduction. Colletotrichum spp. mainly causes anthracnosedisease in hot chili. This fungus damages on branches, leaves,flowers and fruits. The symptoms of anthracnose are circular orangular sunken lesions on hot chili fruits and shaped brownspots with dark brown edges on leaves, with concentric rings ofacervuli. When symptoms are more serious, hot chili fruitsbecome shrinkage and inedible or drop, which can cause 70-80% yield loss. Nowadays, many kinds of fungicides are used tocontrol pathogenic fungi in plants. However, the utilization ofhigh concentration of fungicide disrupts the balance of usefulmicroorganisms in soil. This condition can become goodenvironment for the growth of pathogens and existence ofchemical resistant insect pests. The fungicidal residues inagricultural products and soil cause the groundwater pollution,environmental pollution, especially health hazard of human andanimals. To prevent intentionally using of fungicides, diseasetolerant plants are grown but the result is not effective. Thesestrains are low productivity and instability. Moreover, plantelicitors, used as biological solutions, activate the diseaseresistance mechanism in plants. This solution is becoming atrend in sustainable and green agriculture development. With 2the aim of reducing the use of toxic chemicals and geneticallymodified plants. Chitin and silicon are two common ingredientsin nature. Many studies reported that chitosan, oligochitosan(chitin derivatives), along with silicon and nano silica havebiological activities such as antimicrobial, antifungal andincreasing disease resistance ability in most of plants, they helpplant to secrete enzymes, bioagents for pathogenic prevention,and promote the plant growth and development. Based on the above mentions, “Study to produceoligochitosan-silica nano and investigate the induced systemicresistance against anthracnose disease caused by Colletotrichumspp. on hot chilli (Capsicum frutescens L.)” was carried out.2. The objectives of the thesis - Identify the pathogenicity potential and classificate atspecies level of Colletotrichum spp. isolated from hot chilli inVietnam. - Produce stable oligochitosan-silica nano, whichpotentially highly induced effectively the systemic resistanceagainst anthranose disease on hot chilli, based on thecombination of low molecular weight chitosan with nano silica. - Detect the potential control the anthracnose diseasecaused by C. gloeosporioides and C. truncatum on hot chilli invitro, greenhouse and opened-field conditions of oligochitosan-silica ...